What is orthogonal analysis in chromatography method development and why is it beneficial?

What is orthogonal analysis in chromatography method development and why is it beneficial?

• A. Orthogonal analysis uses the same separation principle to confirm results.
• B. Orthogonal analysis uses a different separation principle or orthogonal column to confirm results; reduces risk of co-elution bias and increases confidence in peak assignment.
• C. Orthogonal analysis is not used in chromatography method development.
• D. Orthogonal analysis increases risk by creating inconsistent results.

Answer: (B)