How does gradient elution affect run time and re-equilibration requirements compared to isocratic?

How does gradient elution affect run time and re-equilibration requirements compared to isocratic?

• A. It typically reduces overall run time and requires re-equilibration after each run.
• B. It increases run time and eliminates re-equilibration.
• C. It has the same run time and no re-equilibration.
• D. It cannot separate late-retained compounds.

Answer: (D)