Explain detector saturation and when it becomes a concern in quantitative LC-UV methods.

Explain detector saturation and when it becomes a concern in quantitative LC-UV methods.

• A. Detectors saturate when the signal exceeds the linear range; results in non-linear response; avoid by diluting samples, choosing a different wavelength, or using a different detector or concentration range.
• B. Saturation occurs only at very low concentrations.
• C. Saturation improves accuracy.
• D. Saturation is only an issue for MS detectors.

Answer: (A)